English
11-9-2 (23)
three-dimensional cell culture of tumor tissue / Rattus norvegicus / ethylnitrosourea-induced glioblastoma (undifferentiated glioma) from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Species:
- Rattus norvegicus (brown rat)
- Tumor tissue:
- ethylnitrosourea-induced glioblastoma (undifferentiated glioma), from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- 2D cell culture isolation method:
- enzymatic (0.25% trypsin)
- Passage number:
- 23
- 3D cell culture production method:
- cultivation in dishes with an ultra-low adhesive coating
- Initial cell concentration:
- 100 thousand cells per mL of medium
- Culture medium:
- DMEM/F12, 10% fetal bovine serum, 1× antibiotic-antimycotic
- 3D culture duration:
- 5 days
- Spheroid diameter:
- 145±42 µm
- Spheroid shape:
- regular, with a distinct edge
- Spheroid fusion tendency:
- medium
- Contamination control:
- bacteria, fungi, mycoplasma not detected
- Cryopreservation medium:
- 90% fetal bovine serum, 10% DMSO
- Karyology:
- 2n=42, range of variation in chromosome number 40-42
- Application area:
- experimental oncology