English
293
cell line / Homo sapiens / epithelial
- Lot number:
- 293-11-21
- Deposit date:
- 19.11.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- 293 [HEK-293] (ATCC)
- Origin:
- Homo sapiens, human embryonic kidney cells
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO : 12. D10S1248: 10,14. D12S391: 19. D13S317: 12,14. D16S539: 13. D18S51: 17,18. D19S433: 15,17.2. D1S1656: 15,17.3. D21S11: 30.2. D22S1045: 16. D2S1338: 19. D2S441: 11,15. D3S1358: 17. D5S818: 8. D6S1043: 11. D7S820: 11,12. D8S1179: 12,14. DYS391: -. FGA: 23. GENDER: X. SE33: 17,20. TH01: 7,9.3. TPOX: 11. vWA: 16,19. Yindel: -
- Tumorigenicity:
- tumorigenic in Balb/c Nu mice
- Phenotypic markers (expressed antigens):
- PD-L1, ERα, ERβ, TUBB3, EMT
- Cell growth properties:
- adherent
- Culture conditions:
- medium – EMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- EMEM, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 95% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology