English
293T
cell line / Homo sapiens / epithelial
- Lot number:
- 293T-11-21
- Deposit date:
- 23.11.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- 293T [HEK-293T] (ATCC)
- Origin:
- Homo sapiens, human embryonic kidney cells
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO :11,12,13. D10S1248: 13,14. D12S391: 19,20,21. D13S317: 12,13. D16S539: 9,13. D18S51: 12,13. D19S433: 16.2,17.2. D1S1656: 14,15,17.3. D21S11: 28,30.2. D22S1045: 15,16. D2S1338: 18,19. D2S441: 11,15. D3S1358: 15,16,17. D5S818: 8,9. D6S1043: 11. D7S820: 11. D8S1179: 12,13. DYS391: -. FGA: 22,23. GENDER: X. SE33: 19,20. TH01: 9.3. TPOX: 11. vWA: 16,19,20. Yindel: -
- Tumorigenicity:
- tumorigenic in Balb/c Nu mice
- Phenotypic markers (expressed antigens):
- PD-L1, ERβ, TUBB3, EMT
- Cell growth properties:
- adherent
- Culture conditions:
- medium – EMEM, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- EMEM, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 95% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology