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8305C
cell line / Homo sapiens / epithelial
- Lot number:
- 8305c-12-20
- Deposit date:
- 10.12.2020
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Origin:
- Homo sapiens, female, 67 years old, thyroid carcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 9, 12. D10S1248: 12, 17. D12S391: 19, 24.3. D13S317: 9. D16S539: 10, 11. D18S51: 13, 14. D19S433: 14. D1S1656: 13, 14. D21S11: 30, 32.2. D22S1045: 15. D2S1338: 17, 25. D2S441: 11, 12. D3S1358: 15. D5S818: 10, 13. D6S1043: 13. D7S820: 8, 10. D8S1179: 11, 14. DYS391: – . FGA: 21, 22. GENDER: X. SE33: 24.2, 27.2. TH01: 6, 7. TPOX: 8. vWA: 4, 16. Yindel: –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or EMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:6
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 85% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms