English
92-1
cell line / Homo sapiens / epithelial-like
- Lot number:
- 92-1-11-21
- Deposit date:
- 22.11.21
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- ECACC
- Origin:
- Homo sapiens, female, 76 years old, uveal melanoma
- Morphology:
- epithelial-like
- Age (number of passages):
- 45 passages
- Species identity control:
- STR profiling CSF1PO : 10,11. D10S1248: 14. D12S391: 20,21. D13S317: 11,12. D16S539: 12. D18S51: 12,13. D19S433: 13,15.3. D1S1656: 15,17.3. D21S11: 30. D22S1045: 15,16. D2S1338: 19,23. D2S441: 11,15. D3S1358: 14,15. D5S818: 9,11. D6S1043: 12. D7S820: 10,11. D8S1179: 15. DYS391: -. FGA: 21,23. GENDER: X. SE33: 29.2,32. TH01: 9,9.3. TPOX: 8,9. vWA: 16. Yindel: -
- Tumorigenicity:
- tumorigenic in Balb/c Nu mice
- Phenotypic markers (expressed antigens):
- PD-L1, ERα, ERβ, TUBB3, CD44,
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 92% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology