English
A-431
cell line / Homo sapiens / epithelial
- Lot number:
- A431-06-24
- Deposit date:
- 20.06.2024
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- CRL-1555 (ATCC)
- Origin:
- Homo sapiens, female, 85 years old, epidermoid carcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 11, 12. D10S1248: 13, 15. D12S391: 18, 23. D13S317: 9, 13. D16S539: 12, 14. D18S51: 13, 17. D19S433: 15, 15.2. D1S1656: 16, 18. D21S11: 28, 30. D22S1045: 15, 16. D2S1338: 17, 20. D2S441: 12, 15. D3S1358: 14. D5S818: 12, 13. D6S1043: 12, 18. D7S820: 10. D8S1179: 13. DYS391: –. FGA: 20. GENDER: X. SE33: 16, 22. TH01: 9. TPOX: 11. vWA: 15, 17. Yindel: –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or DMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:6
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 96% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms