English
A875
cell line / Homo sapiens / spindle-cell
- Lot number:
- A875-10-21
- Deposit date:
- 28.10.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- A875
- Origin:
- Homo sapiens, skin melanoma
- Morphology:
- spindle-cell
- Age (number of passages):
- more than 50 passages
- Species identity control:
- STR profiling CSF1PO : 11,12. D10S1248: 13. D12S391:20,22. D13S317: 8,12. D16S539: 11,12. D18S51: 17. D19S433: 13.2,14. D1S1656: 11,12. D21S11: 28,30.2. D22S1045: 16,17. D2S1338: 20,24. D2S441: 11. D3S1358: 14,15. D5S818: 11,13. D6S1043: 19. D7S820: 8,11. D8S1179: 11. DYS391: -. FGA: 22. GENDER: X. SE33: 15. TH01: 9.3. TPOX: 8,11. vWA: 15,16. Yindel: -
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- PD-L1, ERα, ERβ, TUBB3, EMT
- Mutations:
- BRAF
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 fetal bovine serum 10% 2mM L-glutamine HEPES 1% penicillin (100 U/mL) streptomycin (100 µg/mL) 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 96% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology