English
Astrocytoma grade 3-4, 103
cell line / Oryctolagus cuniculus / brain.
- Animal species:
- Rabbit, Oryctolagus cuniculus.
- Age:
- sexually mature animals.
- Tissue:
- brain.
- Morphology:
- fibroblast-like.
- Carcinogenesis:
- 3-Methylcholanthrene (into the brain).
- Number of passages:
- 20
- Optimal cell number:
- about 8×10⁶
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection control:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Karyology:
- 2n= 44, range of variation in chromosome number.
- Molecular profile:
- activation of cytochromes P450, annexin A1, expression of gamma-glutamyltranspeptidase (GGT), nuclear factor erythroid 2-related factor 2 (Nrf2) (antioxidant responses), aryl hydrocarbon receptor (AhR), ptgs1 (prostaglandin-endoperoxide synthase 1), hmox1 (heme oxygenase 1), ephx1 (epoxide hydrolase 1), mgst2 (microsomal glutathione S-transferase 2, glrx1 (glutaredoxin 1), akr7a3 (aldo-keto reductase 7a3), sult1b1 (sulfotransferase 1b1) (xenobiotic metabolism), estrogenic activity (ER target genes), aryl hydrocarbon hydroxylases (AHH), UGT1A2. smarcb1 (SWI / SNF related, matrix associated, actin dependent regulator of chromatin b1), binds p53 and negatively regulates cell proliferation, bcl10 (B-cell CLL / lymphoma 10), which binds NF-κB and regulates apoptosis, gdnf (glial cell line-derived neurotrophic factor), has an anti-apoptotic function, calca (calcitonin / calcitonin-related polypeptide a), csda (cold shock domain protein A), trib3 (tribbles homolog 3) and hmox1 negatively regulate transcription. Activation of genes involved in calcium metabolism, pH, cell volume, cell number, fatty acid metabolism, erythrocyte and redox homeostasis. Activation of proteins involved in mitochondrial metabolism – peroxiredoxin 1-like protein (LOC678748), cps1 (carbamoyl-phosphate synthetase 1), lrrc59 (leucine-rich repeat containing 59), and mrpl23 (mitochondrial ribosomal protein L23). Activation of the regulation of apoptosis and cell proliferation - peroxiredoxin 1, Jun, hmox1 , which participate in the induction of apoptosis, and cfdp1 (craniofacial development protein 1), which is anti-apoptotic, mbd2 (methyl-CpG-binding protein), which negatively regulates transcription, kdr (kinase insert domain receptor) and avpr1a (arginine vasopressin receptor 1A), which positively regulate cell proliferation. Increase in Cyp2j4, rock 1 (Rho-associated coiled-coil), containing protein kinase, which negatively regulate neuronal apoptosis, stat1 (signal transducer and activator of transcription 1), which plays a role in the induction of apoptosis, bnip3l (BCL2/adenovirus E1B interacting protein 3-like), which is involved in the regulation of apoptosis, anti-apoptotic sh3glb1 (SH3-domain GRB2-like endophilin B1), ei24 (etoposide induced 2.4 mRNA), which negatively regulates cell growth and induces apoptosis, syvn1 (synovial apoptosis inhibitor 1), decrease in pigt (phosphatidylinositol glycan anchor biosynthesis, class T), which is responsible for neuronal apoptosis, aqp2 (aquaporin 2), which regulates water reabsorption and cul1 (cullin 1), which positively regulates ubiquitin-protein ligase activity during the mitotic cell cycle (according to the literature).
- Tumoroids obtained:
- no
- Application area:
- neuro-oncology, cell biology.
- Culture method:
- in vitro monolayer, in vivo orthotopically or subcutaneously (see Appendix 1).
- Culture conditions:
- medium – DMEM with glutamine. IDMI with glutamine. fetal bovine serum 20% passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 60%, 10% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- 5×10⁶ cells/mL in ampoule, liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- obtained in the Neuromorphology Laboratory of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery"
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery".
- Author:
- Research Institute of Human Morphology
- Patent, certificate:
- no
- Intended use:
- these samples are intended for laboratory research only.