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Ehrlich-Lettré ascites carcinoma (EAC
cell line / Mus musculus / undifferentiated carcinoma
- Animal species:
- mouse Mus musculus .
- Origin:
- undifferentiated carcinoma
- Age:
- sexually mature
- Tissue:
- undifferentiated carcinoma
- Morphology:
- epithelial
- Histological diagnosis:
- undifferentiated carcinoma
- Tumoroids obtained:
- no
- Subcutaneous growth:
- yes
- Molecular profile:
- resistant to 6-thioguanine
- Tumorigenicity:
- yes, tumors in mice
- Karyology:
- hyperdiploid (modal number 44, 2C = 40) with characteristic A chromosomal, metacentric and minute markers.
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- 3D cell culture, Immuno-oncology, Oncology, Culture methods by passaging in mice are less attractive because of contamination of the tumor with various host inflammatory cells and the development of legislation restricting the use of ascites tumors..
- Culture method:
- in vitro partially adhesion, in vivo (see Appendix 1).
- In vitro culture conditions:
- medium – RPMI-1640 fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum. (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible. Water permeability is highest at the beginning of S phase and gradually decreases to its lowest value immediately after mitosis.
- Intended use:
- these samples are intended for laboratory research only.