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BT-474
cell line / Homo sapiens / epithelial
- Lot number:
- BT474-11-21
- Deposit date:
- 25.11.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- BT-474 (ATCC)
- Origin:
- Homo sapiens, female, 60 years old, invasive ductal carcinoma of the breast
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 10,11. D10S1248: 15. D12S391: 17,18. D13S317: 11. D16S539: 9,11. D18S51: 13,18. D19S433: 14,16.2. D1S1656: 13,15.3. D21S11: 28,32.2. D22S1045: 14. D2S1338: 19. D2S441: 10,14. D3S1358: 17. D5S818: 11,13. D6S1043: 12,17. D7S820: 9,12. D8S1179: 10,12. DYS391: -. FGA: 22,25. GENDER: X. SE33: 17,25.2. TH01: 7. TPOX: 8. vWA: 15,16. Yindel: -
- Tumorigenicity:
- tumorigenic in Balb/c Nu mice
- Phenotypic markers (expressed antigens):
- PD-L1, ERα, ERβ, TUBB3
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 20%, 10µg/mL insulin, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 90% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology