English
CaCo2-HT29 1:1 (6d) 3D
three-dimensional cell culture of tumor tissue / Homo sapiens / culture 1 – intestinal adenocarcinoma, culture 2 – intestinal adenocarcinoma from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Species:
- Homo sapiens sapiens (human)
- Tumor tissue:
- culture 1 – intestinal adenocarcinoma, culture 2 – intestinal adenocarcinoma, from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Passage number:
- immortalized lines
- 3D cell culture production method:
- cultivation in dishes with an ultra-low adhesive coating
- Initial cell concentration:
- 100 thousand cells per mL of medium
- Cell ratio:
- CaCo2:HT29 1:1
- Culture medium:
- DMEM high glucose, 10% fetal bovine serum, 1× antibiotic-antimycotic
- 3D culture duration:
- 6 days
- Spheroid diameter:
- 154±88 µm
- Spheroid shape:
- irregular, with a distinct edge
- Spheroid fusion tendency:
- medium
- Contamination control:
- bacteria, fungi, mycoplasma not detected
- Cryopreservation medium:
- 90% fetal bovine serum, 10% DMSO
- Application area:
- experimental oncology