English
CHO DG44
cell line / Cricetulus griseus / epithelial
- Lot number:
- CHO DG44-12-21
- Deposit date:
- 21.12.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor PHOTO
- Synonyms and presence in other collections:
- CHO DG44 (?)
- Origin:
- Cricetulus griseus, Chinese hamster ovary cells
- Morphology:
- epithelial
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 96% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology