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Clone M-3 [Cloudman S91 melanoma]
cell line / Mus musculus / epithelial cells
- Lot number:
- M3-06-24
- Deposit date:
- 03.06.2024
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- ATCC CCL-53.1
- Origin:
- melanocyte cell line, isolated from mouse skin, Mus musculus. Clone M-3, which produces melanin, was adapted for cell culture by Y. Yasumura, A.H. Tashjian and G. Sato from Cloudman S91 melanoma of a male (C X DBA) F1 mouse.
- Cell type:
- melanocyte
- Morphology:
- epithelial cells
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, gentamicin (20 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 99% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms