English
Ea.hy926 (2d) 3D
three-dimensional cell culture of tumor tissue / Homo sapiens / hybrid of umbilical vein endothelial cells and A549 lung carcinoma cells from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Species:
- Homo sapiens sapiens (human)
- Tumor tissue:
- hybrid of umbilical vein endothelial cells and lung carcinoma cells A549, from the collection of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", Moscow
- Passage number:
- immortalized line
- 3D cell culture production method:
- cultivation in dishes with an ultra-low adhesive coating
- Initial cell concentration:
- 100 thousand cells per mL of medium
- Culture medium:
- DMEM/F12, 10% fetal bovine serum, 1× antibiotic-antimycotic
- 3D culture duration:
- 2 days
- Spheroid diameter:
- 191±75 µm
- Spheroid shape:
- irregular, with a distinct edge
- Spheroid fusion tendency:
- high
- Contamination control:
- bacteria, fungi, mycoplasma not detected
- Cryopreservation medium:
- 90% fetal bovine serum, 10% DMSO
- Karyology:
- aneuploid line, modal chromosome number 80
- Application area:
- experimental oncology