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Pheochromocytoma PC- 12
cell line / Rattus norvegicus / Pheochromocytoma (neuroendocrine)
- Animal species:
- Brown rat Rattus norvegicus.
- Origin:
- Pheochromocytoma
- Age:
- sexually mature animals.
- Tissue:
- adrenal glands
- Morphology:
- small cells of irregular shape, partly adherent/suspension
- Histological diagnosis:
- Pheochromocytoma (neuroendocrine)
- Carcinogenesis:
- spontaneous
- Tumorigenicity (%):
- 95-100% in Wistar rats
- Optimal cell number:
- about 4×10⁵.
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- catecholamines: dopamine. norepinephrine, nerve growth factor (NGF), do not synthesize epinephrine.
- Karyology:
- 40 chromosomes. 38 autosomes plus XY
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- models for normal differentiation (neurite outgrowths appear), neuro-oncology, cell biology, screening, toxicology
- Culture method:
- in vitro partially adhesion/suspension, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – RPMI-1640. fetal bovine serum 5%. horse serum - 10% (to maintain the preneuronal phenotype) passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 60%, 10% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- Cells reversibly respond to NGF, acquiring a neuronal phenotype on culture flasks coated with collagen 4. Transfection is possible.
- Intended use:
- these samples are intended for laboratory research only.