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Glioma 35(line)
cell line / Rattus norvegicus / glioma
- Animal species:
- Brown rat Rattus norvegicus.
- Origin:
- Glioma 35
- Age:
- newborn animals.
- Tissue:
- brain.
- Histological diagnosis:
- glioma
- Carcinogenesis:
- Ethylnitrosourea (to newborn rats intracerebrally)
- Number of passages:
- 22.
- Average latent period (days):
- 35.
- Tumorigenicity (%):
- 80-100% in Wistar rats, brown rat.
- Optimal cell number:
- about 6×10⁵.
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Karyology:
- 2n=42, range of variation in chromosome number: first clone in the cell population 41-44, second clone 81-86. modal chromosome number 81-82 and number of markers - 6 (differential staining).
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Application area:
- neuro-oncology, cell biology.
- Culture method:
- in vitro monolayer, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – DMEM with glutamine. IDMI with glutamine. fetal bovine serum 10% passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 60%, 10% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- obtained in the Neuromorphology Laboratory of the A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery".
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery".
- Author:
- Research Institute of Human Morphology
- Patent, certificate:
- no
- Intended use:
- these samples are intended for laboratory research only.