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Glioma C6
cell line / Rattus norvegicus / glioma
- Animal species:
- Brown rat Rattus norvegicus.
- Origin:
- glioma
- Age:
- sexually mature animals.
- Tissue:
- brain
- Morphology:
- fibroblast-like
- Histological diagnosis:
- glioma
- Carcinogenesis:
- N-Nitroso-N-Methylurea
- Average latent period (days):
- 27.
- Tumorigenicity (%):
- 95-100% in Wistar rats
- Optimal cell number:
- about 4×10⁵.
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- S-100 protein (increases as cells grow from low density to confluence). glycerophosphate dehydrogenase in response to glucocorticoids. somatotrophin, glucocorticoid receptor
- Karyology:
- 2n. The karyotype is stable within the number of stem cells and corresponds to a normal male karyotype. Three cells with breaks. one with a secondary constriction, one with a dicentric, one with a rearrangement and four with terminal or centromeric associations. Cytogenetic instability of some cell lines has been reported in the literature.
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- neuro-oncology, cell biology, screening, toxicology
- Culture method:
- in vitro partially adhesion/suspension, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – F-12K fetal bovine serum 2.5%. horse serum - 15% (to maintain the preneuronal phenotype) passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- Benda et al. series of passages
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- Transfection is possible. Susceptibility to vaccinia virus, vesicular stomatitis, Orsay (Indiana), vesicular stomatitis, Glasgow (Indiana), herpes simplex virus.
- Intended use:
- these samples are intended for laboratory research only.