English
GliomaU87MG
cell line / Homo sapiens / most likely glioblastoma
- Animal species:
- Human, Homo sapiens.
- Origin:
- most likely glioblastoma
- Age:
- sexually mature.
- Tissue:
- brain
- Morphology:
- epithelial
- Histological diagnosis:
- most likely glioblastoma
- Carcinogenesis:
- spontaneous
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- Isoenzymes AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, PGM3.
- Karyology:
- hypodiploid human cell line with a modal chromosome number of 44, occurring in 48% of cells. The frequency of higher ploidy was 5.9%. Twelve markers were common to all cells, including der(1)t(1.3) (p22.q21), der(16)t(1.16) (p22.p12), del(9) (p13) and nine others. The der(1) marker had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.
- Contamination control:
- Mycoplasma contamination was eliminated in 1975.
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, screening, toxicology
- Culture method:
- in vitro adhesion (see Appendix 1).
- In vitro culture conditions:
- medium – EMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- J. Ponten and associates from 1966 -1969 , Allen, 2016.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible, 3D cell culture, neuroscience, immuno-oncology
- Intended use:
- these samples are intended for laboratory research only.