English
HCT 116
cell line / Homo sapiens / epithelial
- Lot number:
- HCT116-09-23
- Deposit date:
- 25.09.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- HCT 116 CCL-247 (ATCC)
- Origin:
- Homo sapiens, male, colon, colorectal carcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 7, 10. D10S1248: 11, 14, 15. D12S391: 17, 21. D13S317: 10, 12. D16S539: 11, 13. D18S51: 16, 17. D19S433: 12, 13. D1S1656: 12, 14. D21S11: 29, 30. D22S1045: 14, 16. D2S1338: 16. D2S441: 11, 12. D3S1358: 12, 18?, 19. D5S818: 10, 11. D6S1043: 13. D7S820: 11, 12. D8S1179: 12, 14. DYS391: – . FGA: 18, 23. GENDER: X SRY. SE33: 12, 13, 26.2. TH01: 8, 9. TPOX: 8. vWA: 17, 22. Yindel: 2
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms