English
HeLa
cell line / Homo sapiens / epithelial
- Lot number:
- HeLa-12-21
- Deposit date:
- 03.12.2021
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- HeLa CRM-CCL-2 (ATCC)
- Origin:
- Homo sapiens, female, 31 years old, cervical adenocarcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 12. D10S1248: 13, 17. D12S391: 19, 22. D13S317: 11. D16S539: 12. D18S51: 15. D19S433: 14.2. D1S1656: 14, 15. D21S11: 29, 31. D22S1045: 12. D2S1338: 24. D2S441: 10. D3S1358: 16, 17. D5S818: 9. D6S1043: 18. D7S820: 10. D8S1179: 13, 16. DYS391: – . FGA: 21. GENDER: X. SE33: 20. TH01: 6, 9. TPOX: 8. Yindel: – . vWA: 14, 17
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or EMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms