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Hepa 1-6
cell line / Mus musculus / epithelial Mouse line: C57/L
- Lot number:
- Hepa1-6-12-23
- Deposit date:
- 25.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Hepa 1-6 [Hepa1-6] ATCC
- Origin:
- Mus musculus, hepatoma
- Morphology:
- epithelial
- Mouse strain:
- C57/L
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or DMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:4 – 1:8
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 90% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms