English
Huh-7
cell line / Homo sapiens / epithelial-like
- Lot number:
- Huh7-12-23
- Deposit date:
- 25.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- HuH-7. HUH-7. HuH7. Huh7. HUH7. HUH7.0. JTC-39. Japanese Tissue Culture-39. KCLB (Korean Cell Line Bank)
- Origin:
- Homo sapiens, male, 57 years old, hepatocellular carcinoma
- Morphology:
- epithelial-like
- Species identity control:
- STR profiling CSF1PO: 11. D10S1248: 16. D12S391: 20, 21. D13S317: 10, 11. D16S539: 10. D18S51: 15. D19S433: 13, 14. D1S1656: 16. D21S11: 30. D22S1045: 17, 20. D2S1338: 19. D2S441: 12, 14. D3S1358: 15. D5S818: 12. D6S1043: 13, 15. D7S820: 11. D8S1179: 14. DYS391: –. FGA: 22, 23. GENDER: X. SE33: 19, 20, 27.2. TH01: 7. TPOX: 8, 11. vWA: 16, 18. Yindel: –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or DMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:5
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 88% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms