English
Jiyoye
cell line / Homo sapiens / B-lymphoblast
- Lot number:
- Jiyoye-12-21
- Deposit date:
- 8.12.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Jiyoye [Jijoye, P-2003, P-3J] (ATCC)
- Origin:
- Homo sapiens, boy, 7 weeks old, Burkitt lymphoma
- Morphology:
- B-lymphoblast
- Species identity control:
- STR profiling CSF1PO: 10,11. D10S1248: 13,15. D12S391: 18,20. D13S317: 12. D16S539: 10,11. D18S51: 12. D19S433: 12,15. D1S1656: 14,14.3. D21S11: 28,36. D22S1045: 17,18. D2S1338: 21. D2S441: 12,13. D3S1358: 16,17. D5S818: 12. D6S1043: 13,14. D7S820: 8,10. D8S1179: 14,15. DYS391: 10. FGA: 23,24. GENDER: X,Y,SRY. SE33: 18,20. TH01: 7,9. TPOX: 6,8. vWA: 15,19. Yindel: 2
- Tumorigenicity:
- non-tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- CD19, CD20, PD-L1, ERα, ERβ, TUBB3
- Cell growth properties:
- suspension
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 80% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology