English
KMS-12-PE
cell line / Homo sapiens / Multiple myeloma line
Multiple myeloma line derived from the pleural effusion of a 64-year-old woman with refractory terminal multiple myeloma (Ig non-producing) after combination chemotherapy. carries the rearrangement t(11
- Morphology:
- round cells growing singly or occasionally in small clusters in suspension
- Age (number of passages):
- – Species identity control - STR profiling:
- Cell growth properties:
- suspension
- Culture conditions:
- 80% RPMI 1640 + 20% FBS 37 °C, 5% CO2
- Doubling time:
- about 60-80 hours
- Subculture procedure:
- split ratio 1:2 – 1:4
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %Immediately after thawing, viability may drop to 50%, but the cells recover within a few days
- Viability after cryopreservation:
- 80% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biologySafety declarationThe material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms