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Colorectal adenocarcinoma CaCo2
cell line / Homo sapiens / Colorectal adenocarcinoma
- Animal species:
- Human, Homo sapiens.
- Origin:
- Colorectal adenocarcinoma
- Age:
- elderly person
- Tissue:
- large intestine
- Morphology:
- heterogeneous. Apparently, various cell types are present.
- Histological diagnosis:
- Colorectal adenocarcinoma
- Carcinogenesis:
- spontaneous
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- keratin. retinoic acid-binding protein 1. retinol-binding protein 2, heat-stable enterotoxin (Sta, E. coli) and epidermal growth factor (EGF), isoenzymes AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, PGM3.
- Karyology:
- The modal chromosome number of the stemline is 96, occurring in 16% of cases with polyploidy of 3.2%. Ten common markers were found, i.e. t(1q.?), 10q-, t(11q17q) and 7 others. T(1q17q) and M11 were found in some cells. Ins(2), 10q- and t(15q.?) were generally paired, while t (11q.17q) and t(21q.?) were mostly three-copy. Normal N9 was absent, and N21 was lost in some cells. One to 4 small acrocentric chromosomes were found. On Q-banding, no Y chromosome with a bright distal q-band was found.
- Contamination control:
- Mycoplasma contamination was eliminated in 1975.
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, screening, toxicology
- Culture method:
- in vitro partially adhesion/suspension, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – EMEM fetal bovine serum 20%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- J. Ponten and associates from 1966 -1969 , Allen, 2016.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible, 3D cell culture, neuroscience, Susceptibility to human immunodeficiency virus 1. In a monolayer, the cells show signs of enterocytic differentiation.
- Intended use:
- these samples are intended for laboratory research only.