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Lymphoma YAC-1
cell line / Mus musculus / lymphoma
- Animal species:
- mouse Mus musculus
- Origin:
- Lymphoma
- Tissue:
- lymph node
- Morphology:
- lymphoblasts, suspension
- Histological diagnosis:
- lymphoma
- Carcinogenesis:
- Moloney murine leukemia virus (Mo-MuLV).
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Contamination control:
- Mycoplasma contamination was eliminated in 1975.
- Viral infection:
- negative for ectromelia virus (mousepox).
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- oncology, 3D cell culture, immunology, NK cell analysis.
- Culture method:
- in vitro partially adhesion/suspension, in vivo orthotopically or subcutaneously (see Appendix 1).
- In vitro culture conditions:
- medium – RPMI-1640 fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- J. Ponten and associates from 1966 -1969 , Allen, 2016.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- transfection is possible, 3D cell culture, neuroscience, Susceptibility to human immunodeficiency virus 1. In a monolayer, the cells show signs of enterocytic differentiation.
- Intended use:
- these samples are intended for laboratory research only.