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Human umbilical vein cell line EA.hy926
cell line / Homo sapiens / endothelium
- Animal species:
- Human, Homo sapiens.
- Origin:
- endothelium (somatic cell hybrid)
- Age:
- sexually mature
- Tissue:
- endothelium
- Morphology:
- epithelial
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- Factor VIII-related antigen
- Tumorigenicity:
- yes, tumors in nude mice
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- toxicology, cardiovascular diseases
- Culture method:
- in vitro adhesion, colonies in soft agar, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium – DMEM fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- was created by fusion of primary human umbilical vein cells with a thioguanine-resistant clone of A549 (lung carcinoma) by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- suitable for transfection, Electron photomicrographs show the cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated functions of endothelial cells such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation.
- Intended use:
- these samples are intended for laboratory research only.