English
LL/2 (LLC1)
cell line / Mus musculus / round cells
- Lot number:
- LLC1-12-23
- Deposit date:
- 04.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- ATCC CRL-1642
- Origin:
- Mus musculus, Lewis lung carcinoma, isolated from the lung of a C57BL mouse
- Morphology:
- rounded cells
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or DMEM, fetal bovine serum 10%, 2mM L-glutamine, gentamicin (20 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:4 – 1:6
- Cryopreservation:
- RPMI-1640 or DMEM, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms