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LNCaP clone FGC
cell line / Homo sapiens / epithelial
- Lot number:
- LNCaP-12-23
- Deposit date:
- 14.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- LNCaP clone FGC, ATCC
- Origin:
- Homo sapiens, male, 50 years old, prostate carcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 10, 11. D10S1248: 13, 15, 16. D12S391: 21, 22. D13S317: 10, 12. D16S539: 11. D18S51: 9, 11, 12. D19S433: 13.2, 15. D1S1656: 16, 17, 18. D21S11: 29, 31.2, 32.2. D22S1045: 15. D2S1338: 16, 17. D2S441: 11.3. D3S1358: 15, 16. D5S818: 11, 12. D6S1043: 10, 11, 18. D7S820: 9.1, 10.3. D8S1179: 12, 14. DYS391: 10. FGA: 19, 20. GENDER: XY. SE33: 16. TH01: 9. TPOX: 8,9. vWA: 16, 18. Yindel: 2
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:5
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 90% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms