English
mel Hn(2024)
cell line / Homo sapiens / epithelial-like
- Lot number:
- LOT 2024-05-011
- Deposit date:
- 23.09.2024
- Number of cells per ampoule:
- 2,6×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, female, 58 years old, skin melanoma (metastasis) [Human melanoma cell lines. Monograph / Ed. by I.N. Mikhailova and M.M. Davydov. – St. Petersburg: Naukoemkie Tekhnologii, 2012. – 174 pp.]
- Morphology:
- epithelial-like
- Age (number of passages):
- 7 passages
- Species identity control:
- STR profiling CSF1PO: 10, 11. D10S1248: 14. D12S391: 15, 20. D13S317: 9, 13. D16S539: 11,12. D18S51: 17. D19S433: 12, 15. D1S1656: 15, 17. D21S11: 28, 31. D22S1045: 15, 16. D2S1338: 17, 21. D2S441: 11, 11.3. D3S1358: 14, 19. D5S818: 10, 12. D6S1043: 13. D7S820: 10. D8S1179: 13, 14. DYS391: —. FGA: 20. GENDER: X. SE33: 15, 19. TH01: 9.3. TPOX: 8. vWA. 16, 18. Yindel: —
- Karyotype:
- 58~75<3n>[54]/<6n>
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- Melan A, PD-L1, ERα, ERβ, TUBB3, cytokeratin
- Mutations:
- BRAF+, NRAS-, KRAS-, CDKN2A-, PTEN-,TP53-
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:5
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 95% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology Genomic characterization