English
mel Rac
cell line / Homo sapiens / mixed-cell, polymorphic
- Lot number:
- melRac-10-21
- Deposit date:
- 28.10.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, male, 54 years old, skin melanoma (metastasis) [Human melanoma cell lines. Monograph / Ed. by I.N. Mikhailova and M.M. Davydov. – St. Petersburg: Naukoemkie Tekhnologii, 2012. – 174 pp.]
- Morphology:
- mixed-cell, polymorphic
- Age (number of passages):
- 30 passages
- Species identity control:
- STR profiling CSF1PO: 12. D10S1248: 13,15. D12S391: 19,21. D13S317: 8,9. D16S539:11,12. D18S51: 16,17. D19S433: 14,15. D1S1656: 11,15.3. D21S11: 30. D22S1045: 15. D2S1338: 20,25. D2S441: 11,14. D3S1358: 16,17. D5S818: 10. D6S1043: 11. D7S820: 11. D8S1179: 13. DYS391: 10. FGA:21,22. GENDER: XY,SRY. SE33: 23.2,25.2. TH01: 6,9. TPOX: 8,10. vWA: 15,17. Yindel: 2
- Karyotype:
- 70~84<3n>XX,-Y,add(1)(p36),+add(1)(p36),del(6) (q21)×2, i(7)(q10)×2,der(9.14)(q10.q10),+der(?)t(?.1) (?.q12)×2,+2~3mar,inc[22] 70~84<3n>,YY,‑X,+3,add(5)(q35),der(9)add(9)(p21), inc[11]
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- CD63, HMW, PD-L1, ERα, ERβ, TUBB3, EMT
- Mutations:
- NRAS
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL) 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:4
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 96% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology Genomic characterization