English
mel Si
cell line / Homo sapiens / epithelial-like
- Lot number:
- melSi-10-21
- Deposit date:
- 28.10.2021
- Number of cells per ampoule:
- 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- Unique line
- Origin:
- Homo sapiens, female, 46 years old, skin melanoma (metastasis) [Human melanoma cell lines. Monograph / Ed. by I.N. Mikhailova and M.M. Davydov. – St. Petersburg: Naukoemkie Tekhnologii, 2012. – 174 pp.]
- Morphology:
- epithelial-like
- Age (number of passages):
- 40 passages
- Species identity control:
- STR profiling CSF1PO : 11,12. D10S1248: 13,14. D12S391: 19,23. D13S317: 8,11. D16S539: 11. D18S51: 12,18. D19S433: 13,14. D1S1656: 14,18.3. D21S11: 29,31.2. D22S1045: 12,15. D2S1338: 17,20. D2S441: 10,11. D3S1358: 16. D5S818: 11,12. D6S1043: 11. D7S820: 10,12. D8S1179: 14. DYS391: -. FGA: 20,21. GENDER: X. SE33: 19,28.2. TH01: 9.3. TPOX: 8. vWA: 17. Yindel: -
- Karyotype:
- 46~49<2n>,XX,+2,dup(15)(q21q26.2),+2mar,inc
- Tumorigenicity:
- tumorigenic in Balb/c nude mice
- Phenotypic markers (expressed antigens):
- CD63, HMB45, Tyrosinase, PD-L1, ERα, ERβ, TUBB3, EMT
- Mutations:
- BRAF
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640, fetal bovine serum 10%, 2mM L-glutamine, HEPES 1%, penicillin (100 U/mL), streptomycin (100 µg/mL) 37 °C, 5% CO2
- Subculture procedure:
- twice a week, cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:4
- Cryopreservation:
- RPMI-1640, fetal bovine serum 40%, DMSO 7.5 %, ≥1×10⁶ cells per ampoule
- Viability after cryopreservation:
- 98% (trypan blue staining at passage zero)
- Contamination:
- Bacteria, yeast, fungi, protozoa not detected (visual method) Mycoplasma not detected (PCR method) Contamination with HCV, HBV, HIV, EBV, CMV, HHV, HPV viruses not detected (PCR method)
- Application area:
- carcinogenesis, cell biology Genomic characterization