English
Acute promyelocytic leukemia HL-60
cell line / Homo sapiens / peripheral blood
- Animal species:
- Human, Homo sapiens.
- Origin:
- Acute promyelocytic leukemia
- Age:
- sexually mature
- Tissue:
- peripheral blood
- Morphology:
- promyeloblasts/suspension
- Molecular profile:
- Myc oncogene, TNF-alpha, after stimulation with phorbol myristic acid, complement and Fc, Isoenzymes AK-1, 1, ES-D, 1. G6PD, B. GLO-I, 1. Me-2, 1. PGM1, 1 .PGM3, 1
- Tumorigenicity:
- yes, tumors in nude mice
- Karyology:
- the stemline chromosome number is pseudodiploid with the 2S component occurring in 6.2%. Five markers (M2 to M6) were common to most S-metaphases. SD, the number of which varied per cell, were found in all karyotyped metaphases. HSR chromosomes were not found.
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- toxicology, immunology, oncology
- Culture method:
- in vitro, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium – IMDM fetal bovine serum 20%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1). They may accumulate and grow in patches.
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- History:
- S.J. Collins, et al.
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- model of normal differentiation, suitable for transfection, they differentiate spontaneously, and differentiation can be stimulated with butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethyl sulfoxide (DMSO, 1-1.5%), actinomycin D and retinoic acid. Phagocytically active and respond to chemotactic stimuli.
- Intended use:
- these samples are intended for laboratory research only.