English
PC-3
cell line / Homo sapiens / epithelial
- Lot number:
- PC3-12-23
- Deposit date:
- 25.12.2023
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- PC-3, ATCC
- Origin:
- Homo sapiens, male, 62 years old, prostate adenocarcinoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 11. D10S1248: 16. D12S391: 21. D13S317: 11. D16S539: 11. D18S51: 14, 15. D19S433: 14. D1S1656: 12, 16. D21S11: 29, 31.2. D22S1045: 15. D2S1338: 18, 20. D2S441: 10, 11. D3S1358: 16. D5S818: 13. D6S1043: 18. D7S820: 8, 11. D8S1179: 13. DYS391: –. FGA: 24. GENDER: X. SE33: 28.2. TH01: 7. TPOX: 8, 9. vWA: 17. Yindel –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – RPMI-1640 or F-12K, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3 – 1:6
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 85% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms