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RPMI-7951
cell line / Homo sapiens / epithelial
- Lot number:
- RPMI7951-02-20
- Deposit date:
- 25.02.2020
- Number of cells per ampoule:
- at least 1×10⁶
- Material form:
- frozen in cryopreservation medium in liquid nitrogen vapor
- Synonyms and presence in other collections:
- RPMI-7951 HTB-66 (ATCC)
- Origin:
- Homo sapiens, female, 18 years old, skin, melanoma
- Morphology:
- epithelial
- Species identity control:
- STR profiling CSF1PO: 12. D10S1248: 13. D12S391: 18, 23. D13S317: 11, 12. D16S539: 12.n D18S51: 17, 18. D19S433: 13, 15. D1S1656: 13. D21S11: 28, 31.2. D22S1045: 15, 16. D2S1338: 17, 23. D2S441: 11. D3S1358: 16, 18. D5S818: 11. D6S1043: 10, 12. D7S820: 11, 12. D8S1179: 12, 13. DYS391: – . FGA: 27. GENDER: X. SE33: 19, 27.2. TH01: 9, 9.3. TPOX: 8. vWA: 17, 19. Yindel: –
- Cell growth properties:
- adherent
- Culture conditions:
- medium – DMEM, fetal bovine serum 10%, 2mM L-glutamine, penicillin (100 U/mL), streptomycin (100 µg/mL). 37 °C, 5% CO2
- Subculture procedure:
- cell detachment with 0.25 % Trypsin-EDTA solution, split ratio 1:3
- Cryopreservation:
- culture medium, fetal bovine serum 40%, DMSO 7.5 %
- Viability after cryopreservation:
- 97% (trypan blue staining at passage zero)
- Bacteria, yeasts, fungi, protozoa:
- not detected (visual method)
- Mycoplasma:
- not detected (PCR method)
- Application area:
- carcinogenesis, virology, cell biology Safety declaration The material is non-toxic, non-infectious, non-explosive and cannot cause any harm to living organisms