English
Clear cell renal carcinoma Caki-1
cell line / Homo sapiens / kidney
- Animal species:
- Human, Homo sapiens.
- Origin:
- Clear cell carcinoma of the kidney
- Age:
- 49 years old
- Tissue:
- kidney
- Morphology:
- epithelial
- Tumoroids obtained:
- yes
- Subcutaneous growth:
- yes
- Molecular profile:
- blood group antigens I(O). Rh-. HLA A9, B12, Bw35, isoenzymes AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1-2 Me-2, 2 PGM1, 1 PGM3, 1-2
- Tumorigenicity:
- yes, tumors in nude mice, forms clear cell carcinoma corresponding to the primary renal failure. also forms tumors in hamsters treated with steroids.
- Karyology:
- model number = 68. range = 63 to 71. The cell line is human aneuploid, with chromosome number in the triploid range. The Y chromosome is absent. however, loss of the Y chromosome is not unusual for male tumor cell lines. All normal autosomes, except chromosomes N9 and N19, are represented, usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1), which is usually trisomic. Normal chromosome N5, and chromosomes N10 and N16, are usually overrepresented relative to the copy number of other normal chromosomes. Thirteen marker chromosomes were identified: 9 q+, t(1 p.?), t(1 qter>1q21::20), t(1q17q), der(11)t(3.11)(q21.q14), 19 q+, 4q+, 4p+ and others. The chromosome number and general cytogenetic features are consistent with those described by J. Fogh et al., J. Natl. Cancer Inst. (Bethesda) 58: 209, 1977.
- Contamination control:
- not detected
- Viral infection:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Application area:
- toxicology, oncology
- Culture method:
- in vitro, in vivo (see Appendix 1).
- In vitro culture conditions:
- Medium –McCoys 5a Medium Modified fetal bovine serum 10%. passaging procedure - cell detachment using trypsin 0.25 %: Versene 0.02 % (1:3), split ratio 1:3 -1:5 cryopreservation – growth medium 65%, 5% DMSO, 30% fetal bovine serum (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing).
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", ATCC.
- Patent, certificate:
- no
- Note:
- suitable for transfection, metastasizes in the skin, ultrastructural features include numerous microvilli, several filaments, numerous small mitochondria, well-developed Golgi cells and ER, numerous lipid droplets and multilamellar bodies, secondary lysosomes, absence of viral particles.
- Intended use:
- these samples are intended for laboratory research only. '