English
Ependymoma, grade 3-4, 14-60-4
transplantable tissue strain / Rattus norvegicus / Neurinoma, grade 3-4
- Animal species:
- Brown rat Rattus norvegicus.
- Age:
- sexually mature animals.
- Tissue:
- brain.
- Histological diagnosis:
- Neurinoma, grade 3-4 malignancy
- Carcinogenesis:
- Ethylnitrosourea (intraperitoneally to females at week 20 of pregnancy).
- Number of passages:
- 21
- Average latent period (days):
- 25
- Tumorigenicity (%):
- 80-100% in Wistar rats, brown rat, hooded rat.
- Optimal cell number:
- 20 µL of minced tissue, about 8×10⁵ cells.
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection control:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Molecular profile:
- Ki 67 index (Me, %): Mitotic figures (Me, per high-power field):
- Karyology:
- 2n= 42, range of variation in chromosome number.
- Cell line obtained:
- no
- Tumoroids obtained:
- no
- Subcutaneous growth:
- yes
- Application area:
- neuro-oncology, cell biology.
- Culture method:
- transplantation of fresh/thawed tissue orthotopically or subcutaneously (see Appendix 1).
- Culture conditions:
- in vivo tissue fresh or thawed after deep freezing (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing). Histological description:
- History:
- obtained in the Laboratory of Experimental Oncology of the N.N. Burdenko National Medical Research Center of Neurosurgery in the late 60s of the 20th century by Professor L.Ya. Yablonovskaya
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery".
- Author:
- Research Institute of Human Morphology
- Patent, certificate:
- no
- Note:
- intracerebral and subcutaneous growth
- Intended use:
- these samples are intended for laboratory research only.