English
Glioma, 2211
transplantable tissue strain / Rattus norvegicus / Glioma
- Animal species:
- Brown rat Rattus norvegicus.
- Age:
- newborn animals.
- Tissue:
- brain.
- Histological diagnosis:
- Glioma
- Carcinogenesis:
- N-Nitroso-N-Methylurea (to newborn rats intracerebrally)
- Tumorigenicity (%):
- 80-100% in Wistar rats, brown rat, hooded rat.
- Optimal cell number:
- 20 µL of minced tissue, about 8×10⁵ cells.
- Contamination control:
- bacteria, fungi and mycoplasma not detected.
- Viral infection control:
- not performed
- Species identity control:
- karyological, isoenzyme (LDH and G6PD) and immunofluorescence analysis.
- Molecular profile:
- S-100 protein. Ki 67 index (Me, %): Mitotic figures (Me, per high-power field):
- Karyology:
- 2n= 42, range of variation in chromosome number 78-85, modal chromosome number 81-83, number of markers - 20 (differential staining).
- Cloning efficiency:
- 45%
- Cell line obtained:
- no
- Tumoroids obtained:
- no
- Subcutaneous growth:
- yes
- Application area:
- neuro-oncology, cell biology.
- Culture method:
- transplantation of fresh/thawed tissue orthotopically or subcutaneously (see Appendix 1).
- Culture conditions:
- in vivo tissue fresh or thawed after deep freezing (see Appendix 1).
- Physical state of the sample:
- frozen.
- Cryopreservation:
- liquid phase and nitrogen vapor (see Appendix 1).
- Viability after cryopreservation:
- 94% (trypan blue staining after thawing). Histological description:
- History:
- obtained at the A.P. Romodanov Institute of Neurosurgery, National Academy of Medical Sciences of Ukraine
- Collections:
- A.P. Avtsyn Research Institute of Human Morphology, Federal State Budgetary Scientific Institution "B.V. Petrovsky Russian Scientific Center of Surgery", KKKP INC RAS
- Author:
- A.P. Romodanov Institute of Neurosurgery (Zhmareva E.N.)
- Patent, certificate:
- no
- Patents, scientific publications using these samples:
- Experim. oncology 1982. 2: 27., Abstract and dissertation in medicine (14.00.14) on the topic: Induction and experimental study of rat brain tumors Zhmareva Kyiv 1984
- Note:
- intracerebral and subcutaneous growth, i.v. (2×10⁵) tumors in the lungs
- Intended use:
- these samples are intended for laboratory research only.